Jay R. Knutson, C. Combs, A. V. Smirnov, J. Xu, T. Rosales, and L. Davenport. National Institutes of Health, Bethesda, MD
Multiphoton microscopy has become a powerful tool for direct 3D sectioned imaging of tissue fluorescence (intrinsic or extrinsic). We report the development of parabolic mirror detection schemes to enhance SNR nearly tenfold in scattering tissues such as brain. We also show initial results for epi-CARS detection of NAD+ in vesicles, to complement the lifetime-based detection of free and bound NADH in vesicles (and living cells) we have previously reported.
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