The synthesis and spectroscopic characterization of novel organo-bridged silsesquioxane precursor, N,N'-bis(4'-(3-triethoxysilylpropyl-ureido)phenyl)-1,4-quinonenediimine (TSUPQD), will be presented. TSUPQD was prepared from the one-step coupling reaction between the emeraldine form of aniline trimer and triethoxysilylpropyl isocyanate (TESPIC). Subsequently, a series of electroactive organic-inorganic hybrid mesoporous materials have been obtained through surfactant-templated sol-gel reactions of TSUPQD and tetraethyl orthosilicate (TEOS), where cationic surfactant cetyltrimethylammonium bromide (CTAB) was employed as structure-directing agent and acetone was used as co-solvent. Nitrogen sorption and X-ray diffraction data show that the resultant nanostructured hybrid materials exhibited large surface areas, high pore volumes and well-ordered structure. Their electrochemical behaviors as monitored by cyclic voltammogram indicate the enhanced electroactivity compared to nonporous counterparts. Furthermore, the electroactive hybrid materials show to be promising scaffold materials in the tissue engineering applications. The electroactive self-assembled monolayers were evenly attached on the glass substrates, followed by covalent modification with an adhesive oligopeptide cyclic RGD on the aromatic amine terminals. The biocompatibility evaluation from PC12 neuronal cell cultures demonstrate the bio-derivatized substrates supported the cell adhesion and proliferation well. It is noteworthy that electroactive surfaces could stimulate spontaneous neuritogenesis of PC12 cells.