Metabonomics is a complex assessment of the composition and temporal changes of metabolites as a result of various systemic stimuli. Stress is a condition in various grades which can develop into a serious disease state and have an effect on the proper functioning of a living system. The metabolic state of a living system will reflect this in many ways and can be characterized by systematically analyzing a variety of samples (biofluids, tissues, cell-extracts, etc.). We investigated a cohort of young horses following 36+ hours of transport from North Dakota to New Jersey and during their adaptation to a new environment. We took samples of the horses' saliva, as an easy-to-access, yet significant biofluid and used NMR-based metabonomics to identify the effect of environmental stress on their metabolic profiles. For the analysis we also used sophisticated software for the NMR processing, statistical evaluation and principle component (PC) analysis. The poster will present our results and demonstrate how subtle effects and differences in the saliva composition were identified. Besides PC analysis, we also attempted molecular level analysis of several variable components.