This report discusses results of validation of the modified microbial genome/proteome solution to the biological agents point detection and identification by mass spectrometry, as reported earlier, by the author. This model system addresses the limitations in the use of proteomic approach by applying correction or weighted correction to reported proteomes for various bacteria,for avoiding false matches with the biological samples protein biomarkers, obtained from experimental mass spectra.The proteomic approach for bacterial identification uses the microbial proteomes derived from the biochemically sequenced microbial genomes, in conjunction with the protein biomarkers from biological samples experimental mass spectra.This is independent of the specific ionization technique and mass analyzer, as it alleviates the requirements for reproducibility crucial in finger print-based approaches. The validation was done by the use of updated microbial genome derived proteins sequence information, as reported in SWISS PROTEIN SEQUENCE RETRIEVAL SYSTEM(SWISS PROT SRS). Corrections to the genome derived proteome for various bacteria may deal with partial protein sequences, uncharacterized proteins, experimentally obtained posttranslational modifications of proteins, Proteins molecular mass entries for multiple strains for same bacteria. Various generalities for posttranslational modifications of proteins in bacteria may also be used for correcting the bacterial proteins molecular masses reported. The validation has been approached by characterization of various bacteria such as, Escherichia coli, Bacillus megaterium, H.pylori, strain2669, B. subtilis (Strain 168, ATCC) and E. coli (ATTCC 11775). This was done by application of this approach for biological agents point detection and identification by evaluation of biological sample mass spectra data from various laboratories.Biological samples, experimental mass spectra used included, published , positive ion MALDI TOF mass spectra for B. subtilis and E. coli, positive and negative ion MALDI TOF mass spectra for H. pylori.