The Regional Laser and Biomedical Technology Laboratories (RLBL) is a state-of-the-art laser and microscopy resource developing ultrafast laser technology for biophysical and biomedical research. Many of the technologies are open to outside users. Core activities involve two-dimensional infrared spectroscopy used in combination with selective isotopic labeling schemes to probe the structure, dynamics and correlated properties of peptide bonds in proteins. At the single molecule level, investigations of molecular motion on the cell membrane, internalization events, diffusive and directed motion in the cytoplasm, and the aggregation and disaggregation of protein complexes are performed employing both total internal reflection fluorescence microscopy and confocal microscopy. We are supported by the National Center for Research Resources (NCRR) division of the National Institute of Health (NIH) and the University of Pennsylvania.