The chromatographic separation of unknown sample mixtures can be challenging for the HPLC method developer if impurities or degradation products are present in the sample. Peaks of these compounds must be resolved from the peaks of interest, thereby increasing the complexity of the method development. In addition, when several methods need to be developed in a relatively short time, the analyst usually does not have enough time to achieve his goal with adequate accuracy.

ChromSwordAuto method development software integrated with LC, LC-MS and SFC instruments enables a rapid and unattended separation of complex mixtures in the reversed phase, normal phase, and chiral separation mode. In the off-line mode a chromatographer can start from structural formulae and properties of reversed-phase columns or from data of two runs. In the on-line mode the software controls HPLC amd SFC instruments and searches for optimal isocratic or(and) gradient conditions fully automatically.