Monday, May 19, 2008 - 1:50 PM
Medical Arts Building, Rm M-136 (Queensborough Community College)
218

Automated Evaluation of Novel Chiral Columns by SFC Using Standard Chiral Compounds Library

Zainab Pirzada, Michelle Personick, Mirlinda Biba, Xiaoyi Gong, Wes Schafer, and Christopher. J. Welch. Merck & Co. Inc., Rahway, NJ

Many new chiral stationary phases (CSP) are introduced to the market each year. Evaluation of new CSPs is typically haphazard and incomplete. To achieve quick and systematic evaluation of new chiral CSPs, a standardized evaluation workflow was proposed in this presentation using automated SFC screening and a standard library of racemic analytes. The SFC system is capable of automated screening of chiral samples in 96-well plate format using 6 columns and multiple solvents. The standard library of racemic analytes was assembled using a combination of proprietary Merck synthesized and commercial chiral compounds. The library was screened in 96-well plate format on 6 newly available CSPs using the above mentioned automated SFC screening system. The screening results were archived in a structure searchable ACD database. The separation performance of the new CSPs was compared with each other and previously available chiral columns.