Dr. Ramesh Reddy Putheti, Member in American Chemical Society., Owingsmills, MD, Prof. R N Okigbo, Nnamdi Azikiwe University, Awka, PMB 5025, Anambra State,, Nigeria, Madhusoodan sai Advanapu, Vel's college of science, University of Madras, India, and Radha Leburu, S.V University, India.
An environmental monitoring study has be conducted in the clean room areas and controlled areas of a harmaceutical formulation facility in Hyderabad India by using settle plate, surface testing, air sampling and personnel monitoring to isolate and identify the microbial contamination in the area and their source of entry. Environmental samples were collected for the five consecutive days from the clean rooms and controlled areas. The Isolated microorganism was identified as Staphylococcus epidermidis,Staphylococcus xylosus,Staphylococcus haemolyticus,Micrococcus lylae,Micrococcus luteus and Kocuria rosea using API Identification system. Among the isolates identified form the clean room and controlled area are of human organ and it is clear that the major source of contamination is personnel in clean room and controlled area. Based on the identification of the isolates it was concluded that most of the contamination to the clean room and controlled area are passes through the personnel