Angelo Damanti1, Yelena Karaseva1, and Angelo Rossi2. (1) U. S. Food and Drug Administration, Jamaica, NY, (2) York College - City University of New York, Jamaica, NY
The Pesticide Analytical Manual (PAM ) is published by the Food and Drug Administration (FDA) as a repository of the analytical methods used by FDA laboratories in examining food commodities for pesticide residues and chemical contaminants for regulatory purposes. The multiresidue method that is presented in detail is PAM Vol I 302 E1. Most nonionic pesticide residues are extracted from nonfatty foods (like fresh fruit and vegetables) by blending with acetone or water/acetone mixture. Residues are transferred from aqueous acetone to methylene chloride / petroleum ether partitioning. Salt is added to aqueous layer after the first partitioning to aid transfer. Methylene chloride is removed by evaporation and concentration steps in the presence of petroleum ether. Final extract is in acetone. Extract is amenable to analytical testing by many determinative steps including gas chromatography, liquid chromatography and mass spectrometry.