We are introducing OHpak SB400 series semi-micro columns for gel filtration chromatography (GFC) analysis. They have similar calibration curves as our popular SB-800 series. In addition, the SB400 columns have better sensitivity and higher theoretical plate number, while using less sample amount and solvent.

The packing material is polyhydroxymethacrylate gel with controlled surface hydrophilicity and pore distribution. The operational pH range is 3 to 10, and the temperature range is 5 to 70 oC. Water soluble organic solvents such as methanol, acetonitrile and DMF can be used.

SB400 series are available with 4 different exclusion limits ranging from 1,000, to 1,000,000. Specifically, the SB401-4E column with the lowest exclusion limit (1,000) is completely new, and not available in the SB-800 series.

We will introduce basic features of SB400 series and their applications in cationic, anionic, and non-ionic polymers.