The specific silencing of genes through RNA interference by double-stranded RNA is now being studied not only as a means to understand cellular processes, but also as a means of treating patients in the pharmaceutical industry and controlling pests in the agricultural sector. While the protein machinery necessary to cause this phenomenon has been well characterized, it is not always possible to predict the silencing of one specific gene. Using a computer algorithm we developed, we have been able to create maps of gene networks in which such interactions might occur due to sequence homology. One of these networks is now being examined in the nematode Caenorhabditis elegans. Two plasmid constructs have been created. The first targets four genes within the network for silencing through a common hairpin sequence found in each of the genes. The second construct targets just one these genes through sequence homology in an area outside of the hairpin. Both exhibit impaired movement as compared C. elegans fed the control construct or no construct. This suggests that the pattern of silenced genes in similar between the two groups and that the silencing of the one gene with the second construct might have led to the silencing of the other three genes in manner that we could then begin to predict. We are currently examining RNA levels for all four genes with the C.elegans to determine if this is the case and will begin to examine the RNA levels of other genes within the network as well.