The primary purpose of this investigation was to further the understanding of using Henna as a dyestuff. A dyestuff is a colored substance which can be used for staining or coloring other materials. The dyestuff found in henna is lawsone which is an orange pigment present in the leaves of the plant. The research involved the dyeing of: jute, silk, nylon, wool, linen, and a blend of nylon: polyamide (50:50). Each material was investigated individually under specified conditions to determine the most ideal set of conditions required for the material to adsorb lawsone. Uptake studies of lawsone were quantified based on percent change in absorbance of the aqueous henna solution. The parameters investigated were: temperature, duration and concentration. The studies indicate that under comparable conditions, nylon: polyamide (50:50) along with wool had the highest uptake of lawsone of the materials investigated. This study about dyeing with henna will provide more insights on its possible use as a natural agent and, promote its use as a substitute for synthetic dyes as a more environmental friendly, greener approach to dyeing.