Electrospun scaffolds are gaining increasing popularity in tissue engineering applications. We have produced a systematic study of the effects of fiber diameter, spacing, mechanics and orientation on cell morphology, proliferation, and dynamics. We studied both glassy (Polymethylmethacrylate, PMMA) and elastomeric (Polybutadiene) fiberous scaffolds. We measured in vitro both the modulii of the primary culture dermal fibroblast cells and the fibers and correlated the result to images of the integrin receptors and actin fibrils. Proliferation measurements indicated that much larger cell densities could be achieved on oriented substrates, as compared with flat films or randomly oriented electrospun scaffolds. Furthermore, cell dynamics measurements of en masse or single cell migration showed that the cells move at constant velocity on fibers, as opposed to flat surfaces where the velocity decreases as the radial distance from the droplet center increases. The relevance of these findings to wound healing applications will be discussed.