DPA is a useful biological agent, acting as a chelating agent of elements in the body and is a constituents of the redox coenzyme, NAD/ NADH. It is used for the quantitative detection of calcium. It is also a useful analytical indicator for the presence of bacteria spores. Thus the sensitive determination of DPA is very important in many biological processes.

A method for the selective detection of dipicolinic acid (DPA) in the presence of other analogs using a molecular imprinting method and fluorescence detection has been developed in our laboratory. We further explored detection methods by incorporating a fluorophore. The fluorophore is immobilized on an (ITO) plate tethered with a silane coupling agent. DPA is imprinted on the plate using a molecular imprinting technique developed in our laboratory. The process has been shown to be very promising for the selective detection of DPA in the presence of other analogs. Incorporating this fluorophore leads to a more versatile instrumental detection method. Comparison of the process will be made with the original parameters obtained without the fluorophore. Reproducibility selectivity, sensitivity, shelf life, real time analysis and fabrication processes will be described fully in this paper.