The discovery and advances in molecular biology and biotechnology resulted in the newest generations of biologics – DNA vaccines to prevent infectious diseases. DNA vaccines mediate both humoral and cellular immune response and are efficacious even in the presence of maternal antibodies in young individuals. DNA vaccines work by introducing genes of the infectious agents into host cells that encode for an antigen that induces protective immune responses. DNA vaccines can contain one or more antigenic epitope either from virus or bacteria structural proteins. The DNA vaccines can be delivered in various ways: naked DNA injected intramuscularly, attached to a microscopic gold or tungsten beads and pushed into the skin a helium blast, using electric field, or incorporated into live, attenuated non-pathogenic viruses or bacteria. Another exciting aspect of DNA vaccines is their potential use as a therapeutic vaccine. The presentation will highlight the current status of DNA vaccine development, molecular designs, delivery methods, adjuvants, and development and testing of vaccines.