Pyrosequencing is a method used to sequence DNA by detecting the pyrophosphate (PPi) group that is generated when a nucleotide is incorporated into the growing DNA strand in the polymerase reaction. However, this method has an inherent difficulty in accurately deciphering homopolymeric regions of DNA templates. We report here the development of a method to solve this problem by using nucleotide reversible terminators. These nucleotide analogues are modified with a reversible chemical moiety capping the 3'-OH group to temporarily terminate the polymerase reaction. In this way, only one nucleotide is incorporated into the growing DNA strand even in homopolymeric regions. After detecting the PPi for sequence determination, the 3'-OH of the primer extension product is regenerated through different deprotection methods. Using a library of chemical moieties (allyl, 2-nitrobenzyl and azidomethyl) as the reversible groups to cap the 3'-OH of the four nucleotides, we have synthesized three sets of 3'-O-modified nucleotides, as reversible terminators for pyrosequencing. The capping moiety on the 3'-OH of the DNA extension product is efficiently removed after PPi detection by either a chemical method or photolysis. To sequence DNA, templates containing homopolymeric regions are immobilized on Sepharose beads, and then extension�signal detection�deprotection cycles are conducted by using the nucleotide reversible terminators on the DNA beads to unambiguously decipher the sequence of DNA templates. Our results establish that this reversible-terminator-pyrosequencing approach can be potentially developed into a new methodology to accurately determine DNA sequences.