The Scratch assay is a technique used to analyze breast cancer cell migration in vitro. A monolayer of cells is “scratched” and photographed after the scratch is initially made, and then at regular intervals until the scratch is closed. We will perform a number of scratch assays on the MDA-MB231 cell line on various chemical substrates. The differential rates of cell migration will be assessed, possibly correlating with the rate of cancer cell metastasis in vivo.