Metastasis is the spread of disease from one organ or part to another non-adjacent organ or part. Studying cell migration, a step in metastasis, may be a key element in discovering novel therapeutic strategies for controlling invasive tumor cells. In our research, we will use in vitro scratch assay to measure the rate of cell migration of human breast cancer cells. We will investigate the effects that various substrates have on the migration of these cells, including collagen and fibronectin.