In the Fall of 2005, the Department of Chemistry and Biochemistry at the University of Maryland began the roll-out of a new 4-semester introductory chemistry course sequence: a single semester of General Chemistry topics through the basics of equilibrium and acid-base chemistry; followed by two semesters of Organic Chemistry; ending with equilibrium, acid-base, redox and kinetics studies explored in the context of a bio-analytical laboratory program. We will describe individual lab exercises in this POGIL-based course, emphasizing how the concept-driven Team Discussion questions in the POGIL General Chemistry lab are revisited and reinforced in the structure of the bio-analytical experiments.