Tubular nanostructures have attracted increasing interest over the past decade. Their small sizes, large surface areas and unique properties may have many potential applications in separations, material transport and catalysis etc. Recently, the development of a versatile wetting technique has not only made the preparation of nanotubes simpler, it has also broadened the range of materials from carbon to various kinds of polymers. In this study, we synthesized PA66 nanotubes (PNT) and employed them for two different applications. The first involved the addition of PNTs into running buffers for capillary electrophoresis (CE). As the search for low-viscosity polymer solutions with high sieving ability is still an issue in CE separation today, alternative studies have been done on the inclusion of carbon nanotubes to running buffers for separating solutes. However, to our knowledge, no such studies have been carried out on the effect of adding PNTs. We attempted an investigation of the effect of PA66 nanotubes on CE separation of oligonucleotides. The second application involved immobilizing urease onto the PNTs in porous templates. PA66 has long been a popular support for enzyme immobilization, which have the advantages of easy recovery and efficient separation of the enzymes from the reaction medium after the catalytic process. Results obtained using a novel method based on the use of PNT/membrane composite as a filter for urea catalysis are presented.