Microbial contamination of surfaces continues to be a significant problem in not only the medical field but also in the home. Of particular concern is the growth of mold and mildew. Mold/mildew is not just an unsightly problem, but it can also trigger health issues, such as allergies and asthma. Our labs have been investigating substituted bis-alkylaminopyrimidines as a source of novel antimicrobial agents. A number of substituted aminopyridines have been prepared in order to optimize the surprising broad spectrum activity against bacteria and fungi. Given the diverse chemical structures of these analogs, the synthesis of a number of analogs will be described. The antimicrobial activity of these analogs will be detailed along with various application testing which demonstrates their utility as potential antimicrobial agents.